An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples.
نویسندگان
چکیده
Lipid peroxidation products like malondialdehyde, 4-hydroxynonenal and F(2)-isoprostanes are widely used as markers of oxidative stress in vitro and in vivo. This study reports the results of a multi-laboratory validation study by COST Action B35 to assess inter-laboratory and intra-laboratory variation in the measurement of lipid peroxidation. Human plasma samples were exposed to UVA irradiation at different doses (0, 15 J, 20 J), encoded and shipped to 15 laboratories, where analyses of malondialdehyde, 4-hydroxynonenal and isoprostanes were conducted. The results demonstrate a low within-day-variation and a good correlation of results observed on two different days. However, high coefficients of variation were observed between the laboratories. Malondialdehyde determined by HPLC was found to be the most sensitive and reproducible lipid peroxidation product in plasma upon UVA treatment. It is concluded that measurement of malondialdehyde by HPLC has good analytical validity for inter-laboratory studies on lipid peroxidation in human EDTA-plasma samples, although it is acknowledged that this may not translate to biological validity.
منابع مشابه
O-13: Evaluation of Leptin Concentration in Seminal Plasma and Its Correlation with Reactive Oxygen Species, Lipid Peroxidation and Pronuclear Formation in Candidates for ICSI Treatment
Background Leptin, as a key hormone in energy homeostasis, plays an important role in reproduction and male fertility. However, its negative or positive effect on sperm quality and assisted reproduction technique is still controversial. The aim of this study was the comparison of seminal leptin concentration in selected couples with male and female factor for intracytoplasmic microinjection (IC...
متن کاملThe Alterations of Plasma Iipid Peroxidation and erythrocyte Superoxide Dismutase and Glutathione Peroxidase Enzyme Activities During Storage of Blood
Abstract Background and objective: Exposure of red blood cells to oxygen radicals can induce Lipid proxidation, hemoglobin damage and hemolysis of erythrocyte .The present study was designed to determine the alteration of plasma lipid peroxidation and erythrocyte Superoxide Dismutase and Glutathione Peroxidase enzyme activities in stored blood and to find out the quantitative alterations and th...
متن کاملDrought-induced chilling tolerance in cucumber involves membrane stabilisation improved by antioxidant system
We assessed changes in ultrastructure, membrane lipid peroxidation and antioxidant systems for cucumbner seedlings subjected to low temperature stress (day/night temperature of 8 oC /5 oC) that had been either pre-treated with 10% PEG for 2 days or not. We found extensive cell structure damage in the non-treated seedlings, whereas the seedlings pretreated with PEG to simulate drought remain...
متن کاملInvestigating the protective effect of Satureja khuzestanica Jamzad essential oil and carvacrol on lipid peroxidation, antioxidant enzymes, glutathione, and total antioxidant capacity in the plasma of diabetic rats
Background: Type 2 diabetes is one of the main causes of death and its treatment is one of the clinical priorities. This study aimed to evaluate the effect of Satureja khuzistanica essential oil (SKEO) and carvacrol on lipid peroxidation index (MDA), plasma total antioxidant capacity (TAC), and also the activity of antioxidant enzymes. Materials and Methods: The study was experimental in natur...
متن کاملEffect of Flaxseed Supplementation and Concurrent Physical Activity on Total Antioxidant Capacity of the Plasma and the Lipid Peroxidation Index of Overweight Women
Background: Obesity is associated with increased oxidative stress. In addition, exercise can increase oxidative stress effects. However, the use of dietary supplements may moderate these indices. Objective: The present study is conducted to assess the effect of flaxseed supplementation on the total antioxidant capacity and lipid peroxidation after four weeks concurrent training in overweight w...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Free radical research
دوره 44 10 شماره
صفحات -
تاریخ انتشار 2010